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- Title
Delayed chlorophyll accumulation and pigment photodestruction in the epicotyls of dark-grown pea (Pisum sativum).
- Authors
Böddi, Béla; Loudèche, Roland; Franck, Fabrice
- Abstract
A comparison was performed of the tetrapyrrole transformations that occur upon irradiation of epicotyl or leaves of dark-grown Pisum sativum L. (var. Zsuzsi, Hungary). High performance liquid chromatography analysis after continuous or flash-irradiation showed that the biosynthetic pathway from protochlorophyllide (Pchlide) to chlorophyll (Chl) a was markedly slowed down at the step of the reduction of geranylgeranyl(gg)-Chl to dihydrogeranylgeranyl (dhgg)-Chl in epicotyls, whereas phytyl-Chl was synthesized in leaves subjected to the same light treatments. Quantitative pigment analysis during continuous irradiations of different intensities also showed that significant Pchlide photodestruction occurred in epicotyls even under weak light. When both Pchlide and chlorophyllide and/or chlorophylls were present in epicotyls, Pchlide photodestruction was faster under 630-nm light than under 670-nm light, which indicates that this process is most efficiently promoted by Pchlide excitation. Pre-incubation of epicotyl segments with 10 m M ascorbate partly alleviated pigment photodestruction in white light. It is concluded that formation of photoactive Pchlide–Pchlide oxidoreductase complexes is important to prevent fast pigment photooxidation after Pchlide accumulation in the dark.
- Subjects
CHLOROPHYLL analysis; PEAS; SEED irradiation; CHLOROPLAST pigments; PHOTOSYNTHETIC pigments; PORPHYRINS; TETRAPYRROLES; PROTOCHLOROPHYLLIDE; ORGANOMAGNESIUM compounds; HIGH performance liquid chromatography
- Publication
Physiologia Plantarum, 2005, Vol 125, Issue 3, p365
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.2005.00569.x