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- Title
FGFR3 and PIK3CA mutations are involved in the molecular pathogenesis of solar lentigo.
- Authors
Hafner, C.; Stoehr, R.; van Oers, J. M. M.; Zwarthoff, E. C.; Hofstaedter, F.; Landthaler, M.; Hartmann, A.; Vogt, T.
- Abstract
Background Solar lentigines (SL) are frequent benign skin lesions appearing on sun-exposed areas especially in elderly people and therefore represent a hallmark of (photo)aged skin. It has been proposed that SL may subsequently evolve into adenoid seborrhoeic keratosis (SK). However, little is known about the genetic basis of SL. In human SK, FGFR3 and PIK3CA mutations have recently been identified. Objectives To analyse SL for potential FGFR3 and PIK3CA mutations. Methods We screened 30 SL for FGFR3 mutations using a SNaPshot® multiplex assay. For PIK3CA mutations we used direct sequencing of exon 9 and a SNaPshot® assay for the H1047R hotspot mutation (exon 20). Because psoralen plus ultraviolet A (PUVA) lentigines show the V600E BRAF hotspot mutation, we additionally investigated this mutation in SL by allele-specific polymerase chain reaction. Results FGFR3 mutations were detected in five of 30 (17%) SL and PIK3CA mutations in two of 28 (7%) SL. None of 28 SL available for BRAF analysis revealed the V600E mutation. Conclusions Our results suggest that FGFR3 and PIK3CA mutations are involved in the pathogenesis of SL. The occurrence of these mutations in both SL and SK suggests a common genetic basis. Our findings furthermore substantiate previous speculations that UV exposure may be a causative factor for FGFR3 and PIK3CA mutations in human skin.
- Subjects
SKIN injuries; KERATOSIS; GENETIC mutation; POLYMERASE chain reaction; EXONS (Genetics); GENETICS; DISEASES in older people
- Publication
British Journal of Dermatology, 2009, Vol 160, Issue 3, p546
- ISSN
0007-0963
- Publication type
Article
- DOI
10.1111/j.1365-2133.2008.08963.x