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- Title
RAPD Analysis on Genetic Diversity of Pinellia ternata (Thunb.) Breit in Different Populations.
- Authors
LIU, Bo; CHEN, Shuying; YANG, Yanqiong; WANG, Li; HOU, Dianyun
- Abstract
[Objective] To discuss the genetic diversity of Pinellia ternata (Thunb.) Breit in China at molecular level. [Methods] With P. ternatain 23 populations in from 15 provinces as the research materials, DNA of 77 samples were extracted by CTAB method. RAPD-PCR amplification analysis was carried out. And statistical analysis was carried out by DPS software. The Euclidean distance was calculated; and the genetic clustering dendrogram was established. [Results] 4 primers were selected out from 24 primers. And 32 amplified bands were obtained by PCR amplification, of which 28 bands were of polymorphism. Thus, the polymorphism rate was as high as 87.4%. Among the 23 groups, DNA in group I had the highest polymorphism rate in different plants of the same population, which was 73.7%; and DNA in group Lx had the lowest polymorphism rate of 18. 2% . The average polymorphism rate of 23 groups was 44. 0% . The degrees of variation within and among populations were both high. [Conclusions] Both natural and cultivated populations of P. ternate were highly mixed. Although some plant individuals had the same phenotype, the genotype varied according to the composition of genetic materials.
- Subjects
CHINA; WAVE amplification; PLANT growth regulation; DEVELOPMENTAL biology; DEOXYRIBOSE; DNA
- Publication
Medicinal Plant, 2014, Vol 5, Issue 6, p7
- ISSN
2152-3924
- Publication type
Article