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- Title
Active tuberculosis is characterized by an antigen specific and strictly localized expansion of effector T cells at the site of infection.
- Authors
Nemeth, Johannes; Rumetshofer, Rudolf; Winkler, Heide-Maria; Burghuber, Otto C.; Müller, Catharina; Winkler, Stefan
- Abstract
Mycobacterium tuberculosis ( MTB)-specific cytokine responses in the peripheral blood and at the site of infection may differ significantly within the same individual, but the under-lying T-cell subset changes are largely unknown. Here, we measured effector and memory T-cell markers on CD4+ T cells ( CD45 RO, cysteine chemokine receptor ( CCR)7, and CD27) in peripheral blood and at the site of active tuberculosis ( TB). Additionally, T cells were stimulated overnight with purified protein derivative ( PPD) and early secretory antigenic target ( ESAT)-6 to determine which T-cell subset produces MTB-specific interferon ( IFN)-γ. A striking decrease in CCR7 and CD27 expression on T cells was noted at the site of active TB. Likewise, IFN-γ expressing, ESAT-6 specific CD4+ CD45 RO+ CD27− T cells were dramatically increased at the site of infection but were not detectable in peripheral blood. An antigen-specific expansion of differentiated T cells at the site of active TB infection was poorly reflected in peripheral blood. Insight in these changes in MTB-specific effector T cells in different compartments of the body could lead to new approaches for immune-based diagnosis and interventions.
- Publication
European Journal of Immunology, 2012, Vol 42, Issue 11, p2844
- ISSN
0014-2980
- Publication type
Article
- DOI
10.1002/eji.201242678