We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
QUANTITATIVE ASSAY OF PLASMA CARBAMAZEPINE AND 10,11-EPOXYCARBAMAZEPINE BASED ON A LC-MS METHOD.
- Authors
D., Andonie; Szende, Vancea; Z. S., Gall; Alexandra, Grosan; C. E., Vari; Maria-Titica, Dogaru
- Abstract
Background: Performing a bioanalytical study to assess plasma concentrations of carbamazepine and its 10,11-epoxy active metabolite outstands as the cornerstone for monitoring therapeutic efficacy and patient safety. Material and methods: Sigma® standards of carbamazepine and 10,11-epoxy carbamazepine were used for assaying the respective analytes from plasma. In order to obtain the analytical calibration curves, blank human plasma harvested and processed at the Blood Collection Centre of Târgu Mureș was used to prepare the samples. Rabbit and rat blank plasma of blood collected from animals raised in the University s experimental animals breeding station was also used to prepare the samples for generating the respective calibration curves in these species. MassHunter® software for Triple Quad LC/MS by Agilent Technologies® was used to process chromatogram data. Results: The calibration curves ranged from 1.1 to 17.6 mg/ml for carbamazepine and from 0.228 to 5.472 mg/ml for the 10,11-epoxy metabolite respectively. The calibration curves were accurate and precise for the whole calibration interval and the calculated coefficient of determination (R2) was higher than 0.994. Conclusions: The developed analytical method hereby described may be used for the determination of plasma carbamazepine and its 10,11-epoxy metabolite in plasma, and is suitable for performing preclinical and clinical bioanalyitical studies.
- Subjects
CARBAMAZEPINE; EPOXY compound derivatives; LIQUID chromatography-mass spectrometry; BLOOD plasma; METABOLITES
- Publication
Acta Medica Marisiensis, 2016, Vol 62, p83
- ISSN
2068-3324
- Publication type
Article