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- Title
Enhanced Production and Characterization of a Highly Stable Extracellular Protease from an Extreme Halophilic Isolate Salicola marasensis.
- Authors
Khoshnevis, Nika; Rezaei, Shahla; Samaei-Nouroozi, Amene; Amin, Mohsen; Moshfegh, Mahsa; Khoshayand, Mohammad Reza; Faramarzi, Mohammad Ali
- Abstract
Owing to their superior catalytic activity in the extreme conditions, extremozymes have found the potential biotechnological applications for industrial purposes. A robust extracellular protease activity was detected in the culture broth of Salicola marasensis, an extreme halophilic bacterium, after a 48 h-incubation. The effect of different media ingredients in a liquid state fermentation was followed with the aim of improving the enzyme production yield. Fractional factorial and Box-Behnken designs were applied to get a 3.4 fold (from 6.0 to 20.3 U mL-1) improvement of protease production. The distinguishing features of this enzyme were stability at a wide range of pH (5.0-11.0) and temperature (25-60 °C), significant compatibility towards organic solvents, metal ions, chemicals, and surfactants, and hydrolysis of a variety of substrates. The properties of this enzyme can be of tremendous help in terms of the halophilic proteolytic extract's industrial applications.
- Subjects
PROTEASE inhibitors; HALOBACTERIUM; CATALYTIC activity; EXTREMOZYMES; PROTEOLYTIC enzymes
- Publication
Iranian Journal of Pharmaceutical Research, 2018, Vol 17, Issue 4, p1392
- ISSN
1735-0328
- Publication type
Article