We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The Envelope Gene of Hepatitis B Virus Is Implicated in Both Differential Virion Secretion and Genome Replication Capacities between Genotype B and Genotype C Isolates.
- Authors
Haodi Jia; Yanli Qin; Chaoyang Chen; Fei Zhang; Cheng Li; Li Zong; Yongxiang Wang; Jiming Zhang; Jisu Li; Yumei Wen; Shuping Tong
- Abstract
Chronic infection by hepatitis B virus (HBV) genotype C is associated with a prolonged replicative phase and an increased risk of liver cancer, compared with genotype B infection. We previously found lower replication capacity but more efficient virion secretion by genotype C than genotype B isolates. Virion secretion requires interaction between core particles and ENVELOPE proteins. In the present study, chimeric constructs between genotype B and genotype C clones were generated to identify the structural basis for differential virion secretion. In addition to dimeric constructs, we also employed 1.1mer constructs, where the cytomegalovirus (CMV) promoter drove pregenomic RNA transcription. Through transient transfection experiments in Huh7 cells, we found that exchanging the entire envelope gene or just its S region could enhance virion secretion by genotype B clones while diminishing virion secretion by genotype C. Site-directed mutagenesis established the contribution of genotype-specific divergence at codons 108 and 115 in the preS1 region, as well as codon 126 in the S region, to differential virion secretion. Surprisingly, exchanging the envelope gene or just its S region, but not the core gene or 3' S region, could markedly increase intracellular replicative DNA for genotype C clones but diminish that for genotype B, although the underlying mechanism remains to be clarified.
- Subjects
HEPATITIS B; GENOTYPES; ALLELES; VIRUSES; LIVER diseases
- Publication
Viruses (1999-4915), 2017, Vol 9, Issue 4, p62
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v9040062