We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Evaluation of different microsatellite motifs for analysing genetic relationships in cultivated sunflower (<em>Helianthus annuus</em> L.).
- Authors
Dehmer, K. J.; Friedt, W.
- Abstract
A total of 16 different simple sequence repeat motifs and an M13 repeat sequence were used as hybridization probes in order to examine the molecular relationships between two German inbred and eight North American sunflower lines of known pedigree, and to determine the suitability of the individual short tandem repeats for genetic analyses and their occurrence in the sunflower genome. Only the oligonucleotides (ACA)6, (CAT)6, (CATA)5, (GACA)4 and (GATA)4 proved to be well suited for the generation of scorable finger-printing patterns, while the other (microsatellite) sequences were either too abundant or too rare. Although different levels of polymorphism were present in hybridizations with the different simple sequence repeats (SSRs), a clear separation of the German material from the USDA lines was feasible not only by evaluating 20 probe-enzyme combinations, but also by combining every individual probe with four different restriction enzymes. In the American material, the expected relationships could be proved with the single exception of a line that did not group in accordance with its pedigree.
- Subjects
SUNFLOWERS; MICROSATELLITE repeats; SPECIES hybridization; PLANT breeding; GENOMES; GENETICS
- Publication
Plant Breeding, 1998, Vol 117, Issue 1, p45
- ISSN
0179-9541
- Publication type
Article
- DOI
10.1111/j.1439-0523.1998.tb01446.x