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- Title
α-blockade is not effective in decreasing tissue bulk in patients suffering from BPH, an in vitro study.
- Authors
Drewa, Tomasz; Pokrywka, Łukasz; Wolski, Zbigniew; Misterek, Bartosz; Dębski, Robert; Styczyński, Jan
- Abstract
Introduction. Stem cells play an important role in the etiology and development of prostate diseases. CD133 allows discrimination between stem and differentiated cells of the prostatic epithelium. Doxazosin (Dox) induces apoptosis in both the prostatic epithelial and prostatic stromal cells. The aim of study was to assess the influence of doxazosin on cell viability within progenitors and differentiated cells cultured in vitro from prostate epithelium. Material and methods. Prostatic epithelial stem and differentiated cell cultures were acquired from 10 patients suffering from benign prostatic hyperplasia (BPH). Cells were magnetically sorted to obtain pure populations of stem and differentiated cells. Primary co-cultures of stem with differentiated cells and pure CD133 cell cultures were obtained as well. After 14 days, primary cultures were incubated for 12 hours with increasing concentrations of doxazosin, 20, 50 and 80 μ M, respectively. Cell viability was estimated using trypan blue exclusion test. Results. Ninety co-cultures contained both stem cells (CD133+) and differentiated prostatic epithelial cells (CD133-). Forty-one cultures contained only sorted epithelial stem cells (CD133+). Doxazosin significantly decreased cell viability in co-cultures of CD133+ and CD133- cells after 12 hours of incubation when compared to control. There were no significant changes in living cell population within CD133+ cultures after 12 hours of incubation with doxazosin when compared with the control. Conclusions. Doxazosin decreased cell number within co-cultures of stem and differentiated prostatic epithelial cells. Stem/progenitor (CD133+) cells were not sensitive to doxazosin treatment. There is a suspicion that differential influence of doxazosin on progenitor and differentiated cells can be partially responsible for lack of prostate volume decrease after α1-antagonist treatment.
- Subjects
PROSTATE diseases; STEM cells; ETIOLOGY of diseases; DOXAZOSIN; CELL culture
- Publication
Central European Journal of Urology, 2009, Vol 62, Issue 4, p263
- ISSN
0500-7208
- Publication type
Article
- DOI
10.5173/ceju.2009.04.art10upd