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- Title
An autotrophic H<sub>2</sub>-oxidizing, nitrate-respiring, Tc( VII)-reducing A cidovorax sp. isolated from a subsurface oxic-anoxic transition zone.
- Authors
Lee, Ji-Hoon; Fredrickson, James K.; Plymale, Andrew E.; Dohnalkova, Alice C.; Resch, Charles T.; McKinley, James P.; Shi, Liang
- Abstract
Increasing concentrations of H2 with depth were observed across a geologic unconformity and associated redox transition zone in the subsurface at the Hanford Site in south-central Washington, USA. An opposing gradient characterized by decreasing O2 and nitrate concentrations was consistent with microbial-catalysed biogeochemical processes. Sterile sand was incubated in situ within a multilevel sampler placed across the redox transition zone to evaluate the potential for Tc( VII) reduction and for enrichment of H2-oxidizing denitrifiers capable of reducing Tc( VII). H2-driven TcO4− reduction was detected in sand incubated at all depths but was strongest in material from a depth of 17.1 m. A cidovorax spp. were isolated from H2-nitrate enrichments from colonized sand from 15.1 m, with one representative, strain JHL-9, subsequently characterized. JHL-9 grew on acetate with either O2 or nitrate as electron acceptor (data not shown) and on medium with bicarbonate, H2 and nitrate. JHL-9 also reduced pertechnetate ( TcO4−) under denitrifying conditions with H2 as the electron donor. H2-oxidizing A cidovorax spp. in the subsurface at Hanford and other locations may contribute to the maintenance of subsurface redox gradients and offer the potential for Tc( VII) reduction.
- Subjects
AUTOTROPHIC bacteria; OXIDATION-reduction reaction; ANOXIC zones; ELECTROPHILES; DENITRIFYING bacteria; BIOGEOCHEMICAL cycles
- Publication
Environmental Microbiology Reports, 2015, Vol 7, Issue 3, p395
- ISSN
1758-2229
- Publication type
Article
- DOI
10.1111/1758-2229.12263