We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Melastoma malabathricum Ethyl Acetate Fraction Induces Secondary Necrosis in Human Breast and Lung Cancer Cell Lines.
- Authors
Idris, Adi; Zulkipli, Ihsan N.; Zulhilmi, Nurul Ramizah; Lee, Huan F.; Rajabalaya, Rajan; Chee, Lim Y.; Majid, Mohamed; David, Sheba R.
- Abstract
Background: Melastoma malabathricum (MM) is a traditional plant used in the Borneo region. The cytotoxic effects of methanol extracts from MM leaves have been reported in a number of human cancer cell lines. However, the mode of cell death by MM has not been investigated. Objective: We investigated the cytotoxic effects of MM in both human breast and lung cancer cell lines, MCF-7 and A549, respectively, and defined the mode of cell death. Materials and Methods: Cell viability was measured using the 3-(4-, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Annexin-V/propidium iodide (PI) and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was done to determine the mode of cell death. Results: The MTT assay revealed that MM extract had an IC50 of >400 µg/ml on both cell lines at 24 h posttreatment. Flow cytometric and fluorescence microscopy analysis of Annexin-V/PI stained MM-treated cells revealed that the majority of the cells underwent secondary necrosis/late apoptosis. TUNEL assay showed that little to no DNA nicks were present in MM-treated cells, suggesting that cells have undergone secondary necrosis, not late apoptosis, at that time point. Conclusion: MCF-7 and A549 cells undergoes secondary necrosis 24 h post-treatment with MM extract. MM leaf extract could be a potential source for a novel anti-tumor agent for cancer therapy.
- Subjects
MELASTOMATACEAE; PHYTOTHERAPY; THERAPEUTIC use of plant extracts; CANCER cells; CELL lines; FLOW cytometry; CANCER treatment
- Publication
Pharmacognosy Magazine, 2017, Vol 13, pS688
- ISSN
0973-1296
- Publication type
Article
- DOI
10.4103/pm.pm_465_15