We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A simple and efficient non-denaturing FISH method for maize chromosome differentiation using single-strand oligonucleotide probes.
- Authors
Zhu, Minqiu; Du, Pei; Zhuang, Lifang; Chu, Chenggen; Zhao, Han; Qi, Zengjun; Schwarzacher, T.
- Abstract
Single-strand oligonucleotides (SSONs hereafter) as probes are becoming a powerful method of chromosome painting in many species. In this study, nine SSONs ((ACT)10, (ACT)19, Knob-1, Knob-2, Knob-3, CentC69-1, MR68-3, K10-72-1, and TR1-357-2) were developed and used for chromosome identification in 16 maize ( Zea mays L., 2 n = 20) inbred lines and hybrids by non-denaturing fluorescence in situ hybridization (ND-FISH). Each SSON produced clear signals on 2-10 chromosomes of inbred lines B73 and Mo17. A multiplex probe set containing four SSONs ((ACT)10, Knob-2, CentC69-1, and MR68-3) clearly characterized all maize chromosomes in the 16 lines by a single round of ND-FISH and revealed genetic variation at a chromosome level. For example, unique signals on chromosome 6 clearly distinguished all 16 genotypes. The SSONs and multiplex probe developed in this research will facilitate genotype identification and chromosome research in maize.
- Subjects
PLANT chromosomes; NUCLEIC acid probes; GENOTYPES; ALLELES; GENETICS
- Publication
Genome, 2017, Vol 60, Issue 8, p657
- ISSN
0831-2796
- Publication type
Article
- DOI
10.1139/gen-2016-0167