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- Title
Endomorphins 1 and 2 Inhibit IL-10 and IL-12 Production and Innate Immune Functions, and Potentiate NF-κB DNA Binding in THP-1 Differentiated to Macrophage-Like Cells.
- Authors
AZUMA, Y.; OHURA, K.
- Abstract
We evaluated immunological effects of opioid peptides endomorphins 1 and 2 on the production of interleukin-10 (IL-10) and IL-12 cytokines, functions related to innate immunity and NF-κB DNA binding in human cell line THP-1. Endomorphins 1 and 2 inhibited lipopolysaccharide (LPS)-stimulated IL-10 and IL-12 production in THP-1 differentiated to macrophage-like cells by phorbol 12-myristate 13-acetate (PMA). Similarly, they suppressed LPS-stimulated IL-10 and IL-12 production in THP-1 matured to monocytes by 1α,25-dihydroxyvitamin D3 . In addition, endomorphins 1 and 2 led to marked potentiation of NF-κB binding in THP-1 differentiated to macrophage-like cells. Furthermore, these endomorphins further potentiated LPS-induced NF-κB binding. Moreover, they inhibited chemotaxis, phagocytosis of Escherichia coli and PMA-stimulated production of hydrogen peroxide in THP-1 differentiated to macrophage-like cells. These results suggest that endomorphins 1 and 2 may inhibit THP-1 functions, such as cytokine production and functions related to innate immune, and potentiate NF-κB DNA binding in THP-1.
- Subjects
INTERLEUKINS; OPIOID peptides; MACROPHAGES
- Publication
Scandinavian Journal of Immunology, 2002, Vol 56, Issue 3, p260
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1046/j.1365-3083.2002.01128.x