We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Transmission of Retroviral Infection by Transfusion of Seronegative Blood in Nonhuman Primates.
- Authors
Jehuda-Cehen, Tamar; Powell, Jonathan D.; Sell, Kenneth W.; Villinger, Francois; Lockwood,, Ellen; McClure, Harold M.; Ahmed-Ansari, A.
- Abstract
Techniques such as polyclonal B cell activation with pokeweed mitogen (PWM) and polymerase chain reaction (PCR) analysis have documented the existence of simian immunodeficiency virus(SIV)-and human immunodeficiency virus type I-seronegattve but infected humansand nonhuman primates. To establish whether blood from suchseronegative but PWM- and PCR-positive monkeys can transmit infection, naive macaques were transfused with whole blood (n =2) or cultured cells and supernatant fluid (n =2) from two seronegative but PWM- and PCR-positive sooty mangabeys. Aftertransfusion, threeofthefourrecipients seroconverted, and peripheral blood mononuclear cells from all four recipients secreted SIV-reactive antibodies upon polyclonal activation in vitroand were SIV-positive by PCR that used highly specific gag primer pairs and probe. In addition, CD8+ cells from all four recipients markedly inhibited replication of SIV in autologous cells in vitro. These data suggest caution in the sole use of serologic tests forthe detection ofretroviral infection and document the ability ofsuchblood samples to transmit infection.
- Publication
Journal of Infectious Diseases, 1991, Vol 163, Issue 6, p1223
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1093/infdis/163.6.1223