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- Title
Extracting intracellular diffusive states and transition rates from single-molecule tracking data.
- Authors
Persson, Fredrik; Lindén, Martin; Unoson, Cecilia; Elf, Johan
- Abstract
We provide an analytical tool based on a variational Bayesian treatment of hidden Markov models to combine the information from thousands of short single-molecule trajectories of intracellularly diffusing proteins. The method identifies the number of diffusive states and the state transition rates. Using this method we have created an objective interaction map for Hfq, a protein that mediates interactions between small regulatory RNAs and their mRNA targets.
- Subjects
MOLECULE trapping; BAYESIAN analysis; MARKOV processes; PROTEINS; MICRORNA; RNA
- Publication
Nature Methods, 2013, Vol 10, Issue 3, p265
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.2367