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- Title
Crystallographic ab initio protein structure solution below atomic resolution.
- Authors
Rodríguez, Dayté D.; Grosse, Christian; Himmel, Sebastian; González, César; de Ilarduya, Iñaki M.; Becker, Stefan; Sheldrick, George M.; Usón, Isabel
- Abstract
Ab initio macromolecular phasing has been so far limited to small proteins diffracting at atomic resolution (beyond 1.2 Å) unless heavy atoms are present. We describe a general ab initio phasing method for 2 Å data, based on combination of localizing model fragments such as small á-helices with Phaser and density modification with SHELXE. We implemented this approach in the program Arcimboldo to solve a 222-amino-acid structure at 1.95 Å.
- Subjects
MACROMOLECULES; PROTEINS; AMINO acids; ATOMS; CRYSTALLOGRAPHY; ATOMIC structure
- Publication
Nature Methods, 2009, Vol 6, Issue 9, p651
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1365