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- Title
Evaluation of Intracellular Ca<sup>2+</sup> Concentration by Fura 2 Ratiometry in Encystment-induced Colpoda cucullus.
- Authors
SOGAME, Yoichiro; MATSUOKA, Tatsuomi
- Abstract
In Colpoda cucullus, the signaling pathways for encystment induction involving protein phosphorylation have been believed to be triggered by an increase in the intracellular Ca2+ concentration promoted by cell-to-cell mechanical contact due to overpopulation. By means of fura 2 ratiometry, the present study showed that the intracellular Ca2+ concentration was actually elevated when vegetative cells were induced to encyst by being suspended at a high cell density in the presence of external free Ca2+ and suppressed by chelating external Ca2+. This result strongly suggests that an increase in the intracellular Ca2+ concentration caused by an inflow of Ca2+ promoted by cell-to-cell mechanical contact due to overpopulation enhances the rate of encystation in Colpoda cucullus.
- Subjects
COLPODA; ENCYSTMENT; LIFE cycles (Biology); AQUATIC ecology; FURA-2; PROTOZOAN populations; BIOLOGICAL research
- Publication
Acta Protozoologica, 2013, Vol 52, Issue 1, p51
- ISSN
0065-1583
- Publication type
Article
- DOI
10.4467/16890027AP.13.005.0833