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- Title
Field Cancerization in Sporadic Colon Cancer.
- Authors
Soo-Kyung Park; Chang Seok Song; Hyo-Joon Yang; Yoon Suk Jung; Kyu Yong Choi; Dong Hoe Koo; Kyung Eun Kim; Kyung Uk Jeong; Hyung Ook Kim; Hungdai Kim; Ho-Kyung Chun; Dong Il Park
- Abstract
Background/Aims: Aberrant DNA methylation has a specific role in field cancerization. Certain molecular markers, including secreted frizzled-related protein 2 (SFRP2), tissue factor pathway inhibitor 2 (TFPI2 ), N-Myc downstream-regulated gene 4 (NDRG4) and bone morphogenic protein 3 (BMP3), have previously been shown to be hypermethylated in colorectal cancer (CRC). We aim to examine field cancerization in CRC based on the presence of aberrant DNA methylation in normal-appearing tissue from CRC patients. Methods: We investigated promoter methylation in 34 CRC patients and five individuals with normal colonoscopy results. CRC patients were divided into three tissue groups: tumor tissue, adjacent and nonadjacent normal-appearing tissue. The methylation status (positive: methylation level >20%) of SFRP2 , TFPI2 , NDRG4 , and BMP3 promoters was investigated using methylation-specific PCR. Results: The methylation frequencies of the SFRP2 , TFPI2 , NDRG4 and BMP3 promoters in tumor/adjacent/nonadjacent normal-appearing tissue were 79.4%/63.0%/70.4%, 82.4%/53.6%/60.7%, 76.5%/61.5%/69.2%, 41.2%/35.7%/50.0%, respectively. The methylation levels of the SFRP, TFPI2, NDRG4 and BMP3 promoters in tumor tissues were significantly higher than those in normal-appearing tissue (SFRP2, p=0.013; TFPI2, p<0.001; NDRG4, p=0.003; BMP3, p=0.001). No significant correlation was observed between the methylation levels of the promoters and the clinicopathological variables. Conclusions: The field effect is present in CRC and affects both the adjacent and nonadjacent normal-appearing mucosa.
- Subjects
COLON cancer patients; DNA methylation; EPIGENOMICS; NUCLEOTIDE sequence; COLONOSCOPY
- Publication
Gut & Liver, 2016, Vol 10, Issue 5, p773
- ISSN
1976-2283
- Publication type
Article
- DOI
10.5009/gnl15334