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- Title
IN VITRO PLANT REGENERATION OF TWO CUCUMBER (Cucumis sativum L.) GENOTYPES: EFFECTS OF EXPLANT TYPES AND CULTURE MEDIUM.
- Authors
GROZEVA, Stanislava; VELKOV, Nikolay
- Abstract
The effect of different phytohormone concentrations on callusogenesis and organogenesis in two cucumber genotypes were studied. It was established that the rate of plant regeneration depends on genotype, explant type and culture medium. Hypocotyls were found to be more responsive than cotyledons in morphogenesis. In vitro planlet-regenerants have been obtained in hypocotyls explants on culture medium with 1.0 and 2.0 mgL-1 BA for cultivar Gergana and in 1.0 and 3.0 mgL-1 K -- line 15B. Induction of regeneration in cotyledons were established only in cultivar Gergana on culture medium supplemented with 3.0 mgL-1 BA and in combination of 0.5 mgL-1IAA.
- Subjects
PLANTS; REGENERATION (Biology); CUCUMBER genetics; PLANT hormones; HYPOCOTYLS; PLANT morphology; GENOTYPES; IN vitro studies
- Publication
Genetika (0534-0012), 2014, Vol 46, Issue 2, p485
- ISSN
0534-0012
- Publication type
Article
- DOI
10.2298/GENSR1402485G