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- Title
Biotransformation of cinnamyl alcohol to rosavins by non-transformed wild type and hairy root cultures of Rhodiola kirilowii.
- Authors
Grech-Baran, Marta; Sykłowska-Baranek, Katarzyna; Krajewska-Patan, Anna; Wyrwał, Anna; Pietrosiuk, Agnieszka
- Abstract
Non-transformed wild type (NTWT) and hairy root cultures of Rhodiola kirilowii were grown in medium supplemented with 2.5 mM cinnamyl alcohol as a precursor and/or sucrose (1 %) on the day of inoculation or on the 14th day of culture. Rosarin, rosavin, and rosin were produced by NTWT root culture but only rosarin and rosavin by hairy roots. Approximately 80 and 95 % of the glycosides were released into the medium for NTWT and hairy root cultures, respectively. The highest rosavin yield, 505 ± 106 mg/l, was in hairy root culture when cinnamyl alcohol was applied on the day of inoculation with the addition of sucrose on the 14th day of culture. For rosin production, supplementation with cinnamyl alcohol alone on day 14 was more favourable with the highest amount 74 ± 10 mg/l in NTWT root culture. Only traces of rosarin were detected.
- Subjects
BIOTRANSFORMATION in microorganisms; CINNAMYL alcohol dehydrogenase; GUMS &; resins; GLYCOSIDES; SUCROSE
- Publication
Biotechnology Letters, 2014, Vol 36, Issue 3, p649
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-013-1401-5