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- Title
Characterization of a recombinant l-rhamnose isomerase from Dictyoglomus turgidum and its application for l-rhamnulose production.
- Authors
Kim, Yeong-Su; Shin, Kyung-Chul; Lim, Yu-Ri; Oh, Deok-Kun
- Abstract
A putative recombinant enzyme from Dictyoglomus turgidum was characterized and immobilized on Duolite A568 beads. The native enzyme was a 46 kDa tetramer. Its activity was highest for l-rhamnose, indicating that it is an l-rhamnose isomerase. The maximum activities of both the free and immobilized enzymes for l-rhamnose isomerization were at pH 8.0 and 75 °C in the presence of Mn. Under these conditions, the half-lives of the free and immobilized enzymes were 28 and 112 h, respectively. In a packed-bed bioreactor, the immobilized enzyme produced an average of 130 g l-rhamnulose l from 300 g l-rhamnose l after 240 h at pH 8.0, 70 °C, and 0.6 h, with a productivity of 78 g l h and a conversion yield of 43 %. To the best of our knowledge, this is the first report describing the enzymatic production of l-rhamnulose.
- Subjects
CATALYSTS; ENZYMES; PROTEINS; ENZYMOLOGY; ISOMERIZATION
- Publication
Biotechnology Letters, 2013, Vol 35, Issue 2, p259
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-012-1069-2