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- Title
Characterization of the polyketide spore pigment clusterwhiESainStreptomyces aureofaciensCCM3239.
- Authors
Novakova, Renata; Bistakova, Jana; Kormanec, Jan
- Abstract
A spore pigment polyketide gene cluster,whiESa, was cloned fromStreptomyces aureofaciensCCM3239 using a probe from theS. coelicolorA3(2)whiEgene cluster. Sequence analysis of a 4,657-bp DNA fragment revealed five open reading frames with the highest similarity to theS. coelicolorA3(2)whiElocus responsible for spore pigment biosynthesis, with conservation of the size and position of the genes. ThewhiESagene cluster was disrupted by a homologous recombination inS. aureofaciensCCM3239, replacing the most importantwhiESaIIIgene encoding ketosynthase with a thiostrepton resistance gene. The mutation affected spore pigmentation. In contrast to wild-type grey-pink spore pigmentation, the mutant produced white spores, although overall spore morphology was not affected. Transcriptional analysis ofwhiESarevealed two divergently oriented promoters,whiESap1andwhiESap2, upstream of thewhiESaIandwhiESaVIIIgenes, respectively. Both promoters were developmentally regulated inS. aureofaciensCCM3239. They were induced at the late stages of differentiation, during sporulation of aerial hyphae and were dependent upon early sporulation-specific sigma factor sRpoZ and putative transcription factor WhiB. The level of the transcript originating from thewhiESap2promoter was substantially reduced in asigFmutant ofS. aureofaciensCCM3239, indicating its dependence upon the late sporulation sigma factor sF. Comparison of thewhiEpromoters in three different spore pigment polyketide clusters revealed a highly conserved region upstream of the -35 promoter region that may bind a transcriptional regulator.
- Subjects
POLYKETIDES; STREPTOMYCES aureofaciens; NUCLEOTIDE sequence; GENETIC transcription; PROMOTERS (Genetics); GENETIC recombination
- Publication
Archives of Microbiology, 2004, Vol 182, Issue 5, p388
- ISSN
0302-8933
- Publication type
Article
- DOI
10.1007/s00203-004-0720-2