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- Title
Combined Effects of Environmental Concentration of Oxytetracycline and Polystyrene Microplastics on Intestinal Tract of Juvenile Yellow Catfish (Pelteobagrus fulvidraco).
- Authors
Kong Juan; Fan Boya; Yuan Julin; Yu Liqin
- Abstract
In recent years, oxytetracycline (OTC) and polystyrene microplastics (PS-MPs) have been widely detected in aquatic environment. In order to investigate the combined effects of PS-MPs and OTC on intestinal tract of fish, we investigated the effects of OTC and PS-MPs exposure on growth, intestinal structure and intestinal microflora of fish. Juvenile yellow catfish ( Pelteobagrus fulvidraco) were exposed to 500 ng·L-1 OTC (OTC), 100 (low concentration) (MPs-L) and 1 000 μg·L-1(high concentration) (MPs-H) PS-MPs, or their combination (combined MPs-L+OTC, combined MPs-H+OTC) for 28 d. Results showed that OTC and MPs-L alone exposure had no significant effect on the growth, intestinal antioxidant enzyme activities (including superoxide dismutase (SOD) and catalase (CAT)), digestive enzyme activities (including trypsin (TRS), amylase (AMS) and lipase (LPS)), or intestinal flora (including OTU number, alpha diversity, beta diversity, and relative abundance of species composition at phylum level and genus level) of juvenile yellow catfish. However, combined MPs-L+OTC exposure significantly increased SOD and CAT activities, induced intestinal vacuolation and slight loss of intestinal epithelial cells, as well as significantly increased the relative abundance of Proteobacteria as compared to the control group. Intestinal CAT activity was significantly increased in the combined MPs-L+OTC exposure group as compared to the OTC alone exposure group. Moreover, MPs-H alone exposure inhibited the body weight and weight gain rate of juvenile yellow catfish, induced intestinal vacuolation, significantly increased the activities of SOD and CAT, significantly decreased the activities of digestive enzymes TRS and LPS, and significantly decreased the relative abundance of Firmicutes. Compared with the MPs-H group and OTC group, the effects on oxidase activities, digestive enzyme activities, intestinal injury and intestinal flora were further exacerbated in the combined MPs-H+OTC group. In addition, correlation analysis showed that a significant negative correlation between Staphylococcusand body length. Abundance of Cetobacteriumwas positively correlated with the activities of SOD. Aeromonaswas negatively cor- related with the activity of LPS while positively correlated with AMS. Thus, high concentration of PS-MPs alone or combined OTC exposure might affect intestinal digestive enzyme activities through intestinal injury and changes in the intestinal flora, resulting in growth inhibition of juvenile yellow catfish. In addition, the combined intestinal toxicity of PS-MPs and OTC showed significant synergistic effects. The results of this study might provide a new perspective and theoretical basis for ecological risk assessment of antibiotics and microplastics in aquatic environment.
- Subjects
DIGESTIVE enzymes; FLATHEAD catfish; ECOLOGICAL risk assessment; MICROPLASTICS; INTESTINES; OXYTETRACYCLINE
- Publication
Asian Journals of Ecotoxicology, 2023, Vol 18, Issue 1, p426
- ISSN
1673-5897
- Publication type
Article
- DOI
10.7524/AJE.1673-5897.20220124001