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- Title
Cross-Sectional Analysis of the Microbiota of Human Gut and Its Direct Environment in a Household Cohort with High Background of Antibiotic Use.
- Authors
Vu Thi Ngoc, Bich; Ho Bich, Hai; Galazzo, Gianluca; Vu Tien Viet, Dung; Oomen, Melissa; Nghiem Nguyen Minh, Trang; Tran Huy, Hoang; van Doorn, Hindrik Rogier; Wertheim, Heiman F. L.; Penders, John
- Abstract
Comprehensive insight into the microbiota of the gut of humans and animals, as well as their living environment, in communities with a high background of antibiotic use and antibiotic resistance genes is scarce. Here, we used 16S rRNA gene sequencing to describe the (dis)similarities in the microbiota of feces from humans (n = 107), domestic animals (n = 36), water (n = 89), and processed food (n = 74) in a cohort with individual history of antibiotic use in northern Vietnam. A significantly lower microbial diversity was observed among individuals who used antibiotics in the past 4 months (n = 44) compared to those who did not (n = 63). Fecal microbiota of humans was more diverse than nonhuman samples and shared a small part of its amplicon sequence variants (ASVs) with feces from animals (7.4% (3.2–9.9)), water (2.2% (1.2–2.8)), and food (3.1% (1.5–3.1)). Sharing of ASVs between humans and companion animals was not associated with the household. However, we did observe a correlation between an Enterobacteriaceae ASV and the presence of extended-spectrum beta-lactamase CTX-M-group-2 encoding genes in feces from humans and animals (p = 1.6 × 10−3 and p = 2.6 × 10−2, respectively), hinting toward an exchange of antimicrobial-resistant strains between reservoirs.
- Subjects
VIETNAM; HUMAN microbiota; GUT microbiome; BETA lactamases; ANTIBIOTICS; CROSS-sectional method; DRUG resistance in bacteria
- Publication
Microorganisms, 2021, Vol 9, Issue 10, p2115
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms9102115