We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Intracellular Sugar Transporters Facilitate Cellulase Synthesis in Trichoderma reesei Using Lactose.
- Authors
Wang, Haiyan; Pang, Ai-Ping; Li, Bingzhi; Huo, Liujie; Wu, Fu-Gen; Lin, Fengming
- Abstract
Sugar transporters play an important role in the cellulase production of lignocellulose-degrading fungi. Nevertheless, the role and function of these transporters are still unclear. Here we first report intracellular sugar transporters assisting cellulase production in Trichoderma reesei (T. reesei) using lactose. The mRNA levels of sugar transporter genes mfs, gst, and lac1 were substantially upregulated in T. reesei cultivated on lactose, with the most abundant mRNA levels at 24 h as compared to glucose. Moreover, the individual deletion of these sugar transporters significantly inhibited cellulase production, solid cell growth, and sporulation of T. reesei, suggesting they play a supporting role in cellulase production when grown in lactose. Surprisingly, MFS, GST, and LAC1 were mainly localized in the cytoplasm, with MFS and LAC1 in the endoplasmic reticulum (ER), representing the first discovery of intracellular sugar transporters involved in cellulase biosynthesis in lactose culture. The absence of the gene lac1 noticeably inhibited most of the crucial genes related to cellulase production, including cellulase-encoding genes, transcription factors, and sugar transporters, at 24 h, which was fully relieved at 48 h or 72 h, indicating that lac1 affects cellulase production more at the early step. This research advances the understanding of the function of intracellular sugar transporters in fungi, particularly for fungal cellulase production.
- Subjects
CELLULASE; TRICHODERMA reesei; LACTOSE; TRANSCRIPTION factors; SUGAR; ENDOPLASMIC reticulum; CELL growth
- Publication
Biomolecules (2218-273X), 2023, Vol 13, Issue 2, p295
- ISSN
2218-273X
- Publication type
Article
- DOI
10.3390/biom13020295