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- Title
Synergistic combination of cryoprotectants improved freeze‐dried survival rate and viable counts of Lactiplantibacillus plantarum.
- Authors
Ma, Wenlong; Li, Yinghua; Kang, Wenli; Han, Yifeng; Yin, Boxing; Yang, Renqin; Tang, Rongxue; Pan, Lina; Wang, Jiaqi; Li, Wei; Huang, Yujun; Gu, Ruixia
- Abstract
Well‐designed cryoprotectants are crucial for successful probiotic application. In this study, based on literature analysis, characteristics and economic analysis of cryoprotectants, the sucrose (8.2%)–skim milk (8.0%)–inulin (8.2%) combination was proposed and optimised to enhance freeze‐drying resistance of probiotics. Survival, viable cell counts and inactivation rate during storage at 4°C of Lactiplantibacillus plantarum grx16 protected by the combination was 94.9%, 1.89 × 1011 cfu/g and 0.0116. The optimised sucrose–skim milk–polymer prebiotics combination could also effectively protect several other probiotics, with the survival rate being 89.2–95.6%. These results will aid in the rational development of synergistic cryoprotectant formulations for probiotics.
- Subjects
SURVIVAL rate; CRYOPROTECTIVE agents; FREEZE-drying; PROBIOTICS; PREBIOTICS; SKIM milk; SUCROSE
- Publication
International Journal of Dairy Technology, 2024, Vol 77, Issue 2, p348
- ISSN
1364-727X
- Publication type
Article
- DOI
10.1111/1471-0307.13035