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- Title
Whole genome sequencing of the fast-swimming Southern bluefin tuna (Thunnus maccoyii).
- Authors
Xiaomeng Zhao; Yu Huang; Chao Bian; Xinxin You; Xinhui Zhang; Jieming Chen; Min Wang; Cancan Hu; Yun Xu; Junmin Xu; Qiong Shi
- Abstract
The economically important Southern bluefintuna (Thunnus maccoyii) is a world-famous fast-swimming fish, but its genomic information is limited. Here, we performed whole genome sequencing and assembled a draft genome for Southern bluefin tuna, aiming to generate useful genetic data for comparative functional prediction. The final genome assembly is 806.54 Mb, with scaffold and contig N50 values of 3.31 Mb and 67.38 kb, respectively. Genome completeness was evaluated to be 95.8%. The assembled genome contained 23,403 protein-coding genes and 236.1 Mb of repeat sequences (accounting for 29.27% of the entire assembly). Comparative genomics analyses of this fast-swimming tuna revealed that it had more than twice as many hemoglobin genes (18) as other relatively slow-moving fishes (such as seahorse, sunfish, and tongue sole). These hemoglobin genes are mainly localized in two big clusters (termed as "MN" and "LA" respectively), which is consistent with other reported fishes. However, Thr39 of beta-hemoglobin in the MN cluster, conserved in other fishes, was mutated as cysteine in tunas including the Southern bluefin tuna. Since hemoglobins are reported to transport oxygen efficiently for aerobic respiration, our genomic data suggest that both high copy numbers of hemoglobin genes and an adjusted function of the beta- hemoglobin may support the fast-swimming activity of tunas. In summary, we produced a primary genome assembly and predicted hemoglobin-related roles for the fast-swimming Southern bluefintuna.
- Subjects
BLUEFIN tuna; WHOLE genome sequencing; TUNA; FISH locomotion; GENOMES; COMPARATIVE genomics; DNA copy number variations; HEMOGLOBINS
- Publication
Frontiers in Genetics, 2022, Vol 13, p01
- ISSN
1664-8021
- Publication type
Article
- DOI
10.3389/fgene.2022.1020017