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- Title
GC-MS ANALYSIS OF PHYTOCHEMICALS IN HEXANE EXTRACT OF AMARANTHUS TRICOLOR STEM.
- Authors
Kavitha, R.; Priyadharshini, S.; Gandhimathi, R.
- Abstract
Nowadays, the therapeutic properties of plants are very much attention seeking for human health. Herbal medicines and their products are mainly prepared from crude plant extracts, which contain mixture of phytoconstituents. The present study was carried out to evaluate the antioxidant activity of hexane extract of stem part of Amaranthus tricolor and to identify its phytochemicals by Gas chromatography and Mass spectrometry (GC-MS) analysis. Stem part from the Amaranthus tricolor was shade dried and blended into uniform dry powder. Hexane extract was prepared with dried stem powder in Soxhlet apparatus at 40°C for 72 hrs. The hexane extract of stem part of Amaranthus tricolor was tested for its total phenolic and flavonoid content. In vitro antioxidant activity of the extract was determined using ABTS and 2,2- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Gas chromatography-mass spectrometry analysis was performed and phytoconstituents were identified based on comparison of mass spectra in National Institute of Standards and Technology Library (NIST). The present investigation proved that hexane extract of Amaranthus tricolor stem revealed the presence of phytochemicals flavonoids and phenols. The concentrations of total phenolics and total flavonoids in stem of Amaranthus tricolor were found to be 0.11±0.001mg of GAE/g of dry extract and 0.28±0.08mg of QE/g of dry extract respectively. Gas chromatography-mass spectrometry analysis revealed the presence of 39 compounds in hexane extract. From the experimental findings it was concluded that Amaranthus tricolor stem possess good antioxidant capacity.
- Subjects
PHYTOCHEMICALS; GAS chromatography/Mass spectrometry (GC-MS); AMARANTHS
- Publication
Journal of Advanced Scientific Research, 2020, Vol 11, p111
- ISSN
0976-9595
- Publication type
Article