We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Induction of oxidative stress, apoptosis and DNA damage by koumine in Tetrahymena thermophila.
- Authors
Ye, Qiao; Zhang, Chaonan; Wang, Zhenlu; Feng, Yongyong; Zhou, Aiguo; Xie, Shaolin; Xiang, Qiong; Song, Enfeng; Zou, Jixing
- Abstract
Koumine is a component of the Chinese medicinal herb Gelsemium elegans and is toxic to vertebrates. We used the ciliate Tetrahymena thermophila as a model to evaluate the toxic effects of this indole alkaloid in eukaryotic microorganisms. Koumine inhibited T. thermophila growth and viability in a dose-dependent manner. Moreover, this drug produced oxidative stress in T. thermophila cells and expressions of antioxidant enzymes were significantly elevated at high koumine levels (p < 0.05). Koumine also caused significant levels of apoptosis (p < 0.05) and induced DNA damage in a dose-dependent manner. Mitophagic vacuoles were present in cells indicating induction of autophagy by this drug. Expression of ATG7, MTT2/4, CYP1 and HSP70 as well as the MAP kinase pathway gene MPK1 and MPK3 were significantly altered after exposed to koumine. This study represents a preliminary toxicological evaluation of koumine in the single celled eukaryote T. thermophila.
- Subjects
OXIDATIVE stress; APOPTOSIS; DNA damage; TETRAHYMENA thermophila; HERBAL medicine; ANTIOXIDANTS
- Publication
PLoS ONE, 2019, Vol 14, Issue 2, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0212231