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- Title
Storability, post-storage conversion and genetic stability assessment of alginate-encapsulated shoot tips of monopodial orchid hybrid Aranda Wan Chark Kuan 'Blue' × Vanda coerulea Grifft. ex. Lindl.
- Authors
Gantait, Saikat; Sinniah, Uma
- Abstract
An efficient short-term storage system of synthetic seeds, produced using in vitro shoot tips of the monopodial orchid hybrid Aranda Wan Chark Kuan 'Blue' × Vanda coerulea Grifft. ex. Lindl. (AV), was developed. In vitro shoot tips (3-4 mm) were successfully encapsulated, resulting in uniform spherical beads (capsules), using 3 % sodium alginate with 75 mM CaCl·2HO. Maximum (~100 %) conversion (into plantlets with shoot and root) of capsules (or synthetic seeds) was achieved on quarter-strength Murashige and Skoog regrowth medium, while full-strength MS medium was required for effective conversion of non-encapsulated shoot tips. The capsules showed distinct difference in their response to temperature during storage. The conversion efficiency declined upon storage duration at both 4 and 25 °C, with those stored at 25 °C being more tolerant to storage. Capsules stored at 4 °C had rapid deterioration and faced complete death within 160 days while those stored for 200 days at 25 °C showed relatively high conversion (71.6 %). An inter-simple sequence repeats fingerprinting approach, employed on indiscriminately chosen plantlets from converted capsules (following 4 and 25 °C of storage), ensured the post-storage genetic stability.
- Subjects
SYNTHETIC seeds; PLANT shoots; SODIUM alginate; BACTERIAL capsules; HUMAN fingerprints; NUCLEOTIDE sequence
- Publication
Plant Biotechnology Reports, 2013, Vol 7, Issue 3, p257
- ISSN
1863-5466
- Publication type
Article
- DOI
10.1007/s11816-012-0257-9