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- Title
Influence of thin carbonate-containing apatite coating with molecular precursor method to zirconia on osteoblast-like cell response.
- Authors
KANEKO, Hajime; SASAKI, Hodaka; HONMA, Shinya; HAYAKAWA, Tohru; SATO, Mitsunobu; YAJIMA, Yasutomo; YOSHINARI, Masao
- Abstract
The influence of a thin carbonate-containing hydroxyapatite (CA) coating to tetragonal zirconia polycrystal (TZP) on osteoblastlike cell response was investigated. TZP disks were subjected to blasting and acid etching. Thin CA coatings were deposited by the molecular precursor method (TZP-CA). Initial cell adhesion of mouse osteoblast-like cells MC3T3-E1 was enhanced, and marked progress of actin filaments was observed on TZP-CA compared to on TZP. After 3, 5 or 7 days, cell proliferation on TZP-CA was significantly higher than that on TZP. Alkaline phosphatase activity was slightly lower on TZP-CA than on TZP at 7 days, and no difference was observed at 14 or 21 days. At 28 days incubation, collagenous fibers with mineral precipitants accompanied by phosphorous and amino groups were observed. These results indicate that thin CA coating with molecular precursor method offers promise as a means of enhancing cell response, particularly initial adhesion and proliferation of MC3T3-E1 cells.
- Subjects
CARBONATES; APATITE in the body; COATING processes; PIT &; fissure sealants (Dentistry); ZIRCONIUM oxide; OSTEOBLASTS
- Publication
Dental Materials Journal, 2014, Vol 33, Issue 1, p39
- ISSN
0287-4547
- Publication type
Article
- DOI
10.4012/dmj.2013-122