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- Title
Regulation of Reactive Oxygen Species by Nerve Growth Factor but not Bcl-2 as a Novel Mechanism of Protection of PC12 Cells from Superoxide Anion-Induced Death1.
- Authors
Satoh, Takumi; Yamagata, Tomoko; Ishikawa, Yasuyuki; Yamada, Masashi; Uchiyama, Yasuo; Hatanaka, Hiroshi
- Abstract
Although neurotrophins protect PC12 cells and neurons from oxidative stress-induced death, the molecular mechanism of this effect is largely unknown. Xanthine (XA) + xanthine oxidase (XO) increased the production of the superoxide anion (O2) and hydrogen peroxide (H2O2), and the death of PC12 cells. Catalase but not superoxide dismutase (SOD) nor a NO scavenger protected PC 12 cells from death, indicating that H2O2 is the main effector responsible for this cell death. Both nerve growth factor (NGF) and Bcl-2 protected PC12 cells from O2 -induced toxicity. NGF enhanced the production of O2 and suppressed that of H2O2, suggesting that it inhibits the conversion of O2 to H2O2, while Bcl-2 had no such effect. These results suggested that NGF protected the cells from oxidative stress by altering the composition of the reactive oxygen species (ROS) without affecting their total level
- Subjects
REACTIVE oxygen species; NERVE growth factor; NEUROTROPHINS; CYTOKINES; SUPEROXIDES
- Publication
Journal of Biochemistry, 1999, Vol 125, Issue 5, p952
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/oxfordjournals.jbchem.a022374