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- Title
Through-skull fluorescence imaging of the brain in a new near-infrared window.
- Authors
Hong, Guosong; Diao, Shuo; Chang, Junlei; Antaris, Alexander L.; Chen, Changxin; Zhang, Bo; Zhao, Su; Atochin, Dmitriy N.; Huang, Paul L.; Andreasson, Katrin I.; Kuo, Calvin J.; Dai, Hongjie
- Abstract
To date, brain imaging has largely relied on X-ray computed tomography and magnetic resonance angiography, with their limited spatial resolution and long scanning times. Fluorescence-based brain imaging in the visible and traditional near-infrared regions (400-900 nm) is an alternative, but at present it requires craniotomy, cranial windows and skull-thinning techniques, and the penetration depth is limited to 1-2 mm due to light scattering. Here, we report through-scalp and through-skull fluorescence imaging of mouse cerebral vasculature without craniotomy, utilizing the intrinsic photoluminescence of single-walled carbon nanotubes in the 1.3-1.4 μm near-infrared window (NIR-IIa window). Reduced photon scattering in this spectral region allows fluorescence imaging to a depth of >2 mm in mouse brain with sub-10-μm resolution. An imaging rate of ∼5.3 frames per second allows for dynamic recording of blood perfusion in the cerebral vessels with sufficient temporal resolution, providing real-time assessment of a blood flow anomaly in a mouse middle cerebral artery occlusion stroke model.
- Subjects
BRAIN imaging; NEAR infrared radiation; FLUORESCENCE; CRANIOTOMY; LABORATORY mice
- Publication
Nature Photonics, 2014, Vol 8, Issue 9, p723
- ISSN
1749-4885
- Publication type
Article
- DOI
10.1038/nphoton.2014.166