We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Polyalanine - a practical polypeptide mass calibration standard for matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry in positive and negative mode.
- Authors
Gruendling, Till; Sauerland, Volker; Barahona, César; Herz, Corinna; Nitsch, Ute
- Abstract
Rationale Mass calibration in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is currently obtained using mixtures of individual peptides. This procedure has several drawbacks, including laborious preparation, limited shelf life and unequal calibration mass spacing. Polyalanine, a simple to prepare polydisperse molar mass standard, alleviates these problems. Methods Polyalanine is prepared by the typical protocols for biological sample analytics. Results Polyalanine is the first polymeric standard providing abundant signals in both, positive and negative polarity mode with the typical matrices DHB and HCCA. Conclusions Facile MS as well as MS/MS calibration is thus enabled for the first time in both polarity modes by employing this polydisperse standard. Copyright © 2016 John Wiley & Sons, Ltd.
- Subjects
POLYALANINE; POLYPEPTIDES; DESORPTION ionization mass spectrometry; TANDEM mass spectrometry; TANDEM mass spectrometers
- Publication
Rapid Communications in Mass Spectrometry: RCM, 2016, Vol 30, Issue 6, p681
- ISSN
0951-4198
- Publication type
Other
- DOI
10.1002/rcm.7492