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- Title
Quantification of Ceftaroline in Human Plasma Using High-Performance Liquid Chromatography with Ultraviolet Detection: Application to Pharmacokinetic Studies.
- Authors
Alarcia-Lacalle, Ana; Barrasa, Helena; Maynar, Javier; Canut-Blasco, Andrés; Gómez-González, Carmen; Solinís, María Ángeles; Isla, Arantxazu; Rodríguez-Gascón, Alicia
- Abstract
This study was conducted to develop a rapid, simple and reproducible method for the quantification of ceftaroline in plasma samples by high-performance liquid chromatography with ultraviolet detection (HPLC-UV). Sample processing consisted of methanol precipitation and then, after centrifugation, the supernatant was injected into the HPLC system, working in isocratic mode. Ceftaroline was detected at 238 nm at a short acquisition time (less than 5 min). The calibration curve was linear over the concentration range from 0.25 to 40 µg/mL, and the method appeared to be selective, precise and accurate. Ceftaroline in plasma samples was stable at −80 °C for at least 3 months. The method was successfully applied to characterize the pharmacokinetic profile of ceftaroline in two critically ill patients and to evaluate whether the pharmacokinetic/pharmacodynamic (PK/PD) target was reached or not with the dose regimen administered.
- Subjects
HIGH performance liquid chromatography; CEFTAROLINE; PHARMACOKINETICS; LIQUID chromatography-mass spectrometry; CRITICALLY ill
- Publication
Pharmaceutics, 2021, Vol 13, Issue 7, p959
- ISSN
1999-4923
- Publication type
Article
- DOI
10.3390/pharmaceutics13070959