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- Title
ADAMTS-13 activity and von Willebrand factor levels in methylene blue photo-inactivated plasma processed by either the Springe method or an ‘in house’ system.
- Authors
del Río-Garma, J.; Pereira, A.; Arroyo, J. L.; Mateo, J.; Alvarez-Larrán, A.; Martínez, C.; Muncunill, J.; Barbolla, L.
- Abstract
Background and Objectives Methylene blue photo-inactivated plasma (MBPIP) has been reported to be less effective than fresh-frozen plasma (FFP) in the treatment of thrombotic thrombocytopenic purpura, which suggests a reduced content of the von Willebrand factor metalloprotease ADAMTS-13 in MBPIP. Materials and Methods ADAMTS-13 activity and von Willebrand factor antigen (vWF:Ag) levels were measured in plasma before and after photo-oxidation by either the Springe method or a commercial ‘in house’ system as well as in cryoprecipitate-poor plasma (CPP) and FFP (20 units each). Results Levels of ADAMTS-13 activity in MBPIP processed by the Springe method or the commercial ‘in house’ system were comparable to one another and did not significantly differ from levels found in FFP [median (range): 114% (57–139%), 99% (74–123%), and 106% (70–130%), respectively]. ADAMTS-13 activity was significantly reduced in CPP [median (range): 87% (70–107%) as compared with FFP ( P < 0·05). Levels of vWF:Ag decreased after photo-oxidation by both methods. Conclusion In vitro ADAMTS-13 activity was conserved in MBPIP processed by the two photo-oxidation methods analysed and did not significantly differ from levels found in FFP.
- Subjects
PLASMA exchange (Therapeutics); THROMBOTIC thrombocytopenic purpura; VON Willebrand factor; METALLOPROTEINASES; BLOOD plasma; BLOOD transfusion; METHYLENE blue
- Publication
Vox Sanguinis, 2008, Vol 95, Issue 2, p101
- ISSN
0042-9007
- Publication type
Article
- DOI
10.1111/j.1423-0410.2008.01077.x