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- Title
CRISPR RNA-guided activation of endogenous human genes.
- Authors
Maeder, Morgan L; Linder, Samantha J; Cascio, Vincent M; Fu, Yanfang; Ho, Quan H; Joung, J Keith
- Abstract
Short guide RNAs (gRNAs) can direct catalytically inactive CRISPR-associated 9 nuclease (dCas9) to repress endogenous genes in bacteria and human cells. Here we show that single or multiple gRNAs can direct dCas9 fused to a VP64 transcriptional activation domain to increase expression of endogenous human genes. This proof-of-principle work shows that clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems can target heterologous effector domains to endogenous sites in human cells.
- Subjects
HUMAN genes; BACTERIA; PALINDROMIC DNA; CYTOLOGICAL research
- Publication
Nature Methods, 2013, Vol 10, Issue 10, p977
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.2598