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- Title
Dual RMCE for efficient re-engineering of mouse mutant alleles.
- Authors
Osterwalder, Marco; Galli, Antonella; Rosen, Barry; Skarnes, William C.; Zeller, Rolf; Lopez-Rios, Javier
- Abstract
We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.
- Subjects
EMBRYONIC stem cells; GENES; GENETIC mutation; GENETIC code; GENETICS
- Publication
Nature Methods, 2010, Vol 7, Issue 11, p893
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1521