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- Title
Enhancing the Production of D-Mannitol by an Artificial Mutant of Penicillium sp. T2-M10.
- Authors
Duan, Rongting; Li, Hongtao; Li, Hongyu; Tang, Linhuan; Zhou, Hao; Yang, Xueqiong; Yang, Yabin; Ding, Zhongtao
- Abstract
D-Mannitol belongs to a linear polyol with six-carbon and has indispensable usage in medicine and industry. In order to obtain more efficient D-mannitol producer, this study has screened out a stable mutant Penicillium sp. T2-M10 that was isolated from the initial D-mannitol-produced strain Penicillium sp.T2-8 via UV irradiation as well as nitrosoguanidine (NTG) induction. The mutant had a considerable enhancement in yield of D-mannitol based on optimizing fermentation. The production condition was optimized as the PDB medium with 24 g/L glucose for 9 days. The results showed that the production of D-mannitol from the mutant strain T2-M10 increased 125% in contrast with the parental strain. Meanwhile, the fact that D-mannitol is the main product in the mutant simplified the process of purification. Our finding revealed the potential value of the mutant strain Penicillium sp. T2-M10 to be a D-mannitol-producing strain.
- Subjects
MANNITOL; PENICILLIUM; FERMENTATION; GENETIC mutation; NITROSOGUANIDINE
- Publication
Applied Biochemistry & Biotechnology, 2018, Vol 186, Issue 4, p990
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-018-2791-6