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- Title
Enzymatic Syntheses and Applications of Fluorescent Cyclic Dinucleotides.
- Authors
Li, Yao; Ludford, Paul T.; Fin, Andrea; Rovira, Alexander R.; Tor, Yitzhak
- Abstract
Bacterial cyclic dinucleotides (CDNs) play important roles in regulating biofilm formation, motility and virulence. In eukaryotic cells, theses bacterial CDNs are recognized as pathogen‐associated molecular patterns (PAMPs) and trigger an innate immune response. We report the photophysical analyses of a novel group of enzymatically synthesized emissive CDN analogues comprised of two families of isomorphic ribonucleotides. The highly favorable photophysical features of the CDN analogues, when compared to their non‐emissive natural counterparts, are used to monitor in real time the dinucleotide cyclase‐mediated synthesis and phosphodiesterase (PDE)‐mediated hydrolysis of homodimeric and mixed CDNs, providing effective means to probe the activities of two classes of bacterial enzymes and insight into their biomolecular recognition and catalytic features.
- Subjects
DINUCLEOTIDES; MOLECULAR recognition; BACTERIAL enzymes; EUKARYOTIC cells; RIBONUCLEOTIDES; RIBONUCLEOSIDE diphosphate reductase
- Publication
Chemistry - A European Journal, 2020, Vol 26, Issue 27, p6076
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.202001194