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- Title
Increased Plasma Cell-Free DNA Level during HTNV Infection: Correlation with Disease Severity and Virus Load.
- Authors
Jing Yi; Yun Zhang; Yusi Zhang; Ying Ma; Chunmei Zhang; Qi Li; Bei Liu; Zhijia Liu; Jiayun Liu; Xianqing Zhang; Ran Zhuang; Boquan Jin
- Abstract
Cell-free DNA (cf-DNA) in blood represents a promising DNA damage response triggered by virus infection or trauma, tumor, etc. Hantavirus primarily causes two diseases: haemorrhagic fever with renal syndrome (HFRS) and Hantavirus cardiopulmonary syndrome (HCPS), depending on different Hantavirus species. The aim of this study was to evaluate plasma cf-DNA levels in acute phase of HFRS, and to correlate plasma cf-DNA with disease severity and plasma Hanttan virus (HTNV) load. We observed the appearance of cf-DNA in 166 plasma samples from 76 HFRS patients: the plasma cf-DNA levels peaked at the hypotensive stage of HFRS, and then decreased gradually. Until the diuretic stage, there was no significant difference in plasma cf-DNA level between patients and the healthy control. Exclusively in the febrile/hypotensive stage, the plasma cf-DNA levels of severe/critical patients were higher than those of the mild/moderate group. Moreover, the plasma cf-DNA value in the early stage of HFRS was correlated with HTNV load and disease severity. In most of the patients, plasma cf-DNA displayed a low-molecular weight appearance, corresponding to the size of apoptotic DNA. In conclusion, the plasma cf-DNA levels were dynamically elevated during HFRS, and correlated with disease severity, which suggests that plasma cf-DNA may be a potential biomarker for the pathogenesis and prognosis of HFRS.
- Subjects
DEOXYRIBOSE; AMPLIFIED fragment length polymorphism; MEDICAL microbiology; CONNECTIVE tissue cells; LYMPHOID tissue
- Publication
Viruses (1999-4915), 2014, Vol 6, Issue 7, p2723
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v6072723