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- Title
Protective effects of selenocystine against γ-radiation-induced genotoxicity in Swiss albino mice.
- Authors
Kunwar, Amit; Jayakumar, S.; Bhilwade, H.; Bag, P.; Bhatt, H.; Chaubey, R.; Priyadarsini, K.
- Abstract
Selenocystine (CysSeSeCys), a diselenide aminoacid exhibiting glutathione peroxidase-like activity and selective antitumor effects, was examined for in vivo antigenotoxic and antioxidant activity in Swiss albino mice after exposure to a sublethal dose (5 Gy) of γ-radiation. For this, CysSeSeCys was administered intraperitoneally (i.p.) to mice at a dosage of 0.5 mg/kg body weight for 5 consecutive days prior to whole-body γ-irradiation. When examined in the hepatic tissue, CysSeSeCys administration reduced the DNA damage at 30 min after radiation exposure by increasing the rate of DNA repair. Since antigenotoxic agents could alter the expression of genes involved in cell cycle arrest and DNA repair, the transcriptional changes in p53, p21 and GADD45α were monitored in the hepatic tissue by real-time PCR. The results show that CysSeSeCys alone causes moderate induction of these three genes. However, CysSeSeCys pretreatment resulted in a suppression of radiation-induced enhancement of p21 and GADD45α expression , but did not affect p53 expression. Further analysis of radiation-induced oxidative stress markers in the same tissue indicated that CysSeSeCys significantly inhibits lipid peroxidation and prevents the depletion of antioxidant enzymes and glutathione (GSH) levels. Additionally, it also prevents radiation-induced DNA damage in other radiation sensitive cellular systems like peripheral leukocytes and bone marrow, which was evident by a decrease in comet parameters and micronucleated polychromatic erythrocytes (mn-PCEs) frequency, respectively. Based on these observations, it is concluded that CysSeSeCys exhibits antigenotoxic effects, reduces radiation-induced oxidative stress, and is a promising candidate for future exploration as a radioprotector.
- Subjects
AMINO acids; ANTINEOPLASTIC agents; DNA damage; CELL cycle; DNA repair; POLYMERASE chain reaction; ANTIOXIDANTS; ERYTHROCYTES; OXIDATIVE stress; GENE expression
- Publication
Radiation & Environmental Biophysics, 2011, Vol 50, Issue 2, p271
- ISSN
0301-634X
- Publication type
Article
- DOI
10.1007/s00411-011-0352-2