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- Title
Surface-enhanced Raman scattering assessment of DNA from leaf tissues adsorbed on silver colloidal nanoparticles.
- Authors
Muntean, C. M.; Leopold, N.; Halmagyi, A.; Valimareanu, S.
- Abstract
In this work, the surface-enhanced Raman scattering (SERS) spectra of seven genomic DNAs from leaves of chrysanthemum ( Dendranthema grandiflora Ramat.), common sundew ( Drosera rotundifolia L.), edelweiss ( Leontopodium alpinum Cass), Epilobium hirsutum L., Hypericum richeri ssp. transsilvanicum (Čelak) Ciocârlan, rose ( Rosa x hybrida L.) and redwood ( Sequoia sempervirens D. Don. Endl.), respectively, have been analyzed in the wavenumber range 200-1800 cm−1. The surface-enhanced Raman vibrational modes for each of these cases, spectroscopic band assignments and structural interpretations of genomic DNAs are reported. A high molecular structural information content can be found in the SERS spectra of these DNAs from leaf tissues. Based on this work, specific plant DNA-ligand interactions or accurate local structure of DNA might be further investigated using surface-enhanced Raman spectroscopy. Besides, this study will generate information which is valuable in the development of label-free DNA detection for chemical probing in living cell. Copyright © 2013 John Wiley & Sons, Ltd.
- Subjects
SERS spectroscopy; TISSUE analysis; SILVER nanoparticles; DNA; CHRYSANTHEMUMS; PLANT cells &; tissues
- Publication
Journal of Raman Spectroscopy, 2013, Vol 44, Issue 6, p817
- ISSN
0377-0486
- Publication type
Article
- DOI
10.1002/jrs.4265