We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Morphological-metabolic analysis in Streptomyces rimosus microparticle-enhanced cultivations (MPEC).
- Authors
Ścigaczewska, Anna; Boruta, Tomasz; Bizukojć, Marcin
- Abstract
Streptomyces produce a broad spectrum of biologically active molecules such as oxytetracycline and rimocidin, which are widely used in human and animal treatments. microparticle-enhanced cultivation (MPEC) is one of the tools used for Streptomyces bioprocesses intensification by the control of mycelial morphology. In the present work, morphological changes of Streptomyces rimosus caused by the addition of 10 µm talc microparticles in MPEC were correlated with the biosynthetic activity of the microorganism. Comparing the runs with and without microparticles, major morphological changes were observed in MPEC, including the deformation of pellets, variation of their size, appearance of hyphae and clumps as well as the aggregation of mycelial objects. The presence of talc microparticles also influenced the levels of the studied secondary metabolites produced by S. rimosus. Comparing control and MPEC runs, the addition of talc microparticles increased the amounts of oxytetracycline (9-fold), 2-acetyl-2-decarboxamido-oxytetracycline (7-fold), milbemycin A3+4[O] (3-fold) and CE 108 (1.5-fold), while rimocidin (27-ethyl) and milbemycin β11+4[O] production was reduced. In summary, the addition of talc microparticles to S. rimosus cultivations led to the development of smaller morphological forms like hyphae and clumps as well as to the changes in the amounts of secondary metabolites.
- Subjects
STREPTOMYCES; ANIMAL welfare; METABOLITES; TALC; OXYTETRACYCLINE; RAMAN scattering
- Publication
Bioprocess & Biosystems Engineering, 2024, Vol 47, Issue 6, p891
- ISSN
1615-7591
- Publication type
Article
- DOI
10.1007/s00449-024-03015-2