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- Title
Delayed autoregulation of glucose transport in vascular endothelial cells.
- Authors
Alpert, E.; Gruzman, A.; Riahi, Y.; Blejter, R.; Aharoni, P.; Weisinger, G.; Eckel, J.; Kaiser, N.; Sasson, S.
- Abstract
Aims/hypothesis: We aimed to characterise the development of autoregulation of glucose transport in vascular endothelial cells and its relationship to 12-lipoxygenase (12-LO) expression. Methods: Bovine aortic endothelial cells were exposed to 5.5 and 23.0 mmol/l glucose for up to 48 h. The rates of glucose transport, GLUT-i and i2-LO expression and of i2-hydroxyeicosatetraenoic acid (i2-FIETE) production were determined. Results: We showed high glucose-dependent downregulation of glucose Iransport and Iransporter in vascular endothelial cells within 36-48 h. A similar time-dependent increase in the expression of i 2-LO and the generation of its product i2-HETE was also observed. This downregulatory process was prevented when lip oxygenase ac- tivity was inhibited. Conclusions/interpretation: Vascular endothelial cells, which were previously thought to be "glucose-blind", do in fact downregulate GLUT-i expres- sion and the rate of glucose transport in response to ex- tended exposure to high glucose concentrations. This slow development of glucose-induced downregulation in vascu- lar endothelial cells is related to the slower basal rate of glucose transport in these cells and the slow induction of i2-LO. These data are interesting in view of cuffent hypotheses that attribute vascular endothelial cell dysfunction in diabetes to the lack of a glucose-induced autoregulatory response.
- Subjects
VASCULAR smooth muscle; VASCULAR endothelium; CELLS; GLUCOSE; DIABETES; LIPOXYGENASES
- Publication
Diabetologia, 2005, Vol 48, Issue 4, p752
- ISSN
0012-186X
- Publication type
Article
- DOI
10.1007/s00125-005-1681-y