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- Title
Çevresel Kirleticiler ve Plasental Transporterlar: PCB ile SLC ve ABCB1 Örneği.
- Authors
DİKMEN, Begüm YURDAKÖK; UYAR, Recep; KUZUKIRAN, Özgür; ÜNAL, Mehmet Altay; ÇELİK, Hasan Tolga; BOZTEPE, Ümmü Gülsüm; ALKAN, Kübra KARAKAŞ; ÖZYÜNCÜ, Özgür; BİRER, Yağmur TURGUT; SEVGİLİ, Hilal ÖZDAĞ; KANCA, Halit; AKTAN, Çağdaş; FİLAZİ, Ayhan
- Abstract
Objective: Due to widespread presence and exposure to environmental pollutants, structural and functional changes in membrane transporters could occur, leading to plasental transport of these compounds. In this study, the effects of PCBs on SLC abd ABCB1 membrane transport molecules were evaluated. Materials and Methods: Hemochorial structured human placenta and endotheliochorial structured dog placenta were analyzed for 28 pollutants (PCB, PBDE, PAH and Organochlorines) using GC-MS. The expression profile of the placental whole genome were investigated with RNAseq, and in silico (molecular chelation) and in vitro (SLC and ABCB1 mRNA expression in the placental cell line HTR8/SVneo treated with PCB 101, PCB118) were evaluated. Results: PCB101 826.4μg/kg in one sample out of 60 samples tested; In 23 samples, PCB118 was found to be between 0.14 and 41.9μg/kg. In the bioinformatics findings, there were differences in 742 genes between PCB positive and negative samples in 55 samples that were sequenced (p<0.05). Among these genes, expression differences were found in 14 SLC and 2 ABC families according to groups. The first two genes with the lowest p values in SLC were SLC4A1 (p=0.000126) and SLC38A5 (p=0.000563), while ABCA8 (p=0.022997) and ABCC1 (p=0.025288) were found in ABCs. According to the qPCR findings, there was an increase in mRNA expression for both ABCB1 and SLC22A6, but this increase was found to be higher for ABCB1 (p<0.05). Conclusion: ABCB1 gene expression and binding affinities to PCB ligands were also higher than SLC (SLC1A5). Placental PCB exposure affected ABCB1 expression more than the tested SLCs.
- Subjects
POLYCHLORINATED biphenyls analysis; COMPUTER simulation; IN vitro studies; POLLUTANTS; SEQUENCE analysis; ANIMAL experimentation; GAS chromatography; BIOINFORMATICS; GENE expression; PLACENTA; MASS spectrometry; GENOMES; MESSENGER RNA; DESCRIPTIVE statistics; CELL lines; POLYMERASE chain reaction; POLYCHLORINATED biphenyls; CARRIER proteins; LIGANDS (Biochemistry)
- Publication
Balikesir Health Sciences Journal / Balıkesir Sağlık Bilimleri Dergisi, 2022, Vol 11, p29
- ISSN
2146-9601
- Publication type
Article
- DOI
10.53424/balikesirsbd.1159774