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- Title
Feasibility of Vascular Endothelial Growth Factor Imaging in Human Atherosclerotic Plaque Using <sup>89</sup>Zr-Bevacizumab Positron Emission Tomography.
- Authors
Golestani, Reza; Zeebregts, Clark J.; van Scheltinga, Anton G. T. Terwisscha; Lub-de Hooge, Marjolijn N.; van Dam, Gooitzen M.; Glaudemans, Andor W. J. M.; Dierckx, Rudi A. J. O.; Tio, René A.; Suurmeijer, Albert J. H.; Boersma, Hendrikus H.; Nagengast, Wouter B.; Slart, Riemer H. J. A.
- Abstract
Intraplaque angiogenesis is associated with the occurrence of atherosclerotic plaque rupture. Cardiovascular molecular imaging can be used for the detection of rupture-prone plaques. Imaging with radiolabeled bevacizumab, a monoclonal anti-vascular endothelial growth factor (VEGF)-A, can depict VEGF levels corresponding to the angiogenic status in tumors. We determined the feasibility of 89Zr-bevacizumab imaging for the detection of VEGF in carotid endarterectomy (CEA) specimens. Five CEA specimens were coincubated with 89Zr-bevacizumab and aspecific 111In-labeled IgG to determine the specificity of bevacizumab accumulation. In 11 CEA specimens, 89Zr-bevacizumab micro-positron emission tomography (PET) was performed following 2 hours of incubation. Specimens were cut in 4 mm wide segments and were stained for VEGF and CD68. In each segment, the mean percent incubation dose per gram of tissue (%lnc/g) and tissue to background ratio were determined. A 10-fold higher accumulation of 89Zr-bevacizumab compared to 111ln-lgG uptake was demonstrated by gamma counting. The mean %lnc/ghot spot was 2.2 ± 0.9 with a hot spot to background ratio of 3.6 ± 0.8. There was a significant correlation between the segmental tissue to background uptake ratio and the VEGF score (ρ = .74, p < .001). It is feasible to detect VEGF tissue concentration within CEA specimens using 89Zr-bevacizumab PET. 89Zr-bevacizumab accumulation in plaques is specific and correlates with immunohistochemistry scores.
- Subjects
VASCULAR endothelial growth factors; MEDICAL imaging systems; ATHEROSCLEROTIC plaque; FEASIBILITY studies; ZIRCONIUM isotopes; BEVACIZUMAB; POSITRON emission tomography
- Publication
Molecular Imaging, 2013, Vol 12, Issue 4, p235
- ISSN
1535-3508
- Publication type
Article
- DOI
10.2310/7290.2012.00034