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- Title
Heterologous Expression of Thermogutta terrifontis Endo-Xanthanase in Penicillium verruculosum, Isolation and Primary Characterization of the Enzyme.
- Authors
Denisenko, Yury A.; Korotkova, Olga G.; Zorov, Ivan N.; Rozhkova, Alexandra M.; Semenova, Margarita V.; Elcheninov, Alexandr G.; Kublanov, Ilya V.; Sinitsyn, Arkady P.
- Abstract
Heterologous endo-xanthanase (EX) from the thermophilic planktomycete Thermogutta terrifontis strain was obtained using Penicillium verruculosum 537 (ΔniaD) expression system with the cellobiohydrolase 1 gene promoter. Homogeneous EX with a molecular weight of 23.7 kDa (pI 6.5) was isolated using liquid chromatography methods. This xanthan degrading enzyme also possesses the enzymatic activity towards CM-cellulose, β-glucan, curdlan, lichenan, laminarin, galactomannan, xyloglucan but not towards p-nitrophenyl derivatives of β-D-glucose, mannose and cellobiose. The temperature and pH optima of EX were 55°C and 4.0, respectively; the enzyme exhibited 90% of its maximum activity in the temperature range 50-60°C and pH 3-5.
- Subjects
PENICILLIUM; GALACTOMANNANS; CELLULOSE 1,4-beta-cellobiosidase; ENZYMES; MOLECULAR weights; LIQUID chromatography
- Publication
Biochemistry (00062979), 2021, Vol 86, Issue 4, p489
- ISSN
0006-2979
- Publication type
Article
- DOI
10.1134/S000629792104009X