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- Title
Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases.
- Authors
Toshihiko Katoh; Kenji Yamamoto
- Abstract
Most functional biopharmaceuticals such as antibodies are glycoproteins carrying N-linked oligosaccharides (N-glycans). In animal cells, these glycans are generally expressed as heterogeneous glycoforms that are difficult to separate into a pure form. The structure of these glycans directly affects several biological aspects of the glycoproteins, especially binding affinity. Therefore, the preparation of glycoproteins with well-defined and homogeneous glycoforms is necessary for functional studies and improved efficacy, particularly for biopharmaceuticals. This review describes the recent remarkable progress in the development and production of biopharmaceutical glycan-modified antibodies, through the use of glycan remodeling using microbial endoglycosidases and sophisticated glycoengineering techniques utilizing microbial enzymatic reaction mechanisms.
- Subjects
BIOPHARMACEUTICS; ENDOGLYCOSIDASES; GLYCOPROTEINS; IMMUNOGLOBULIN G; GLYCANS
- Publication
Journal of Applied Glycoscience, 2021, Vol 68, Issue 1, p1
- ISSN
1344-7882
- Publication type
Article
- DOI
10.5458/jag.jag.JAG-2020_0013