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- Title
Recombinant SSP4 protein fromTrypanosoma cruziamastigotes regulates nitric oxide production by macrophages.
- Authors
Ramos-Ligonio, A.; López-Monteon, A.; Talamás-Rohana, P.; Rosales-Encina, J. L.
- Abstract
Acute infection withTrypanosoma cruziis characterized by immunosuppression mediated by T cells and macrophages (Mφs). Nitric oxide (NO) production during the initial phase of acute infection might participate in the clearance of parasites by Mφs, whereas its overproduction during the late phase of acute infection would account for the immunosuppression observed.Trypanosoma cruzimolecules that might regulate the host responses have not been fully identified. Here, we demonstrate that active immunization withMBP::SSP4, a recombinant protein derived from a surface antigen specific ofT. cruziamastigotes (TcSSP4), was able to stimulate Ab production (IgG1, IgG2a, and IgG2b). On the other hand,MBP::SSP4was able to stimulate NO production by peritoneal Mφs from BALB/c mice and Mφs from the J774 cell line. This effect was also observed at the level of inducible nitric oxide synthase (iNOS) detected by Western Blot. Furthermore,MBP::SSP4was also shown to induce the expression of IL-1α, IL-6, IL-12, IFN-γ, and TNF-α in normal animals, and IL-10 in immunized animals. In addition the proteinMBP::SSP4was able to bind to the surface of PMφs and J774 Mφs. These results suggest thatTcSSP4 could modulate Mφ NO production and this may represent a mechanism participating in the immunoregulatory processes during Chagas’ disease.
- Subjects
RECOMBINANT proteins; TRYPANOSOMA cruzi; PARASITES; CYTOKINES; IMMUNOSUPPRESSION; NITRIC oxide; IMMUNOLOGY; CHAGAS' disease
- Publication
Parasite Immunology, 2004, Vol 26, Issue 10, p409
- ISSN
0141-9838
- Publication type
Article
- DOI
10.1111/j.0141-9838.2004.00729.x